The present study was designed to investigate the thrombolytic activity of chloroform extract of leaves of Dioscorea bulbifera. Thrombolytic effect of the fraction was investigated in clot lysis experiment. The extract exerted 31.30% lysis of the blood clot in thrombolytic activity test while 85.77%. And 4.71% lyses were obtained for positive control (streptokinase) and negative control respectively. So, the extract possessed considerable thrombolytic activity which compounds are responsible for the present pharmacological actions and to know their mechanism of action, extensive pharmacological and phytochemical experiments are essential.