Post-polio syndrome (PPS) is a condition that affects polio survivors from an initial acute attack of poliomyelitis virus. It is mainly characterised by new weakening in muscles that were previously affected by the polio infection. The aim of the study was to determine the awareness in medical practitioners regarding post-polio syndrome. A cross sectional survey was conducted in Ahmedabad among 70 practitioners coming across polio survivors. Convenience sampling was used. The medical practitioners were asked to fill a self-administered questionnaire concerning their profile and regarding causes, symptoms, management of PPS. Collected data was analyzed, that gave a brief idea of awareness of PPS among medical practitioners in Ahmedabad. 61% medical practitioners were aware about the term post-polio syndrome. Awareness about clinical features was 54%, about management 48% with only 32% being aware of the role of rehabilitation in PPS.