Introduction: Complicated Urinary Tract Infection (UTI) occurs in patients with structural or functional abnormalities in the genitor-urinary tract. Each patient should be assessed carefully to ensure a correct diagnosis so that anti-microbial therapy is appropriately prescribed for symptomatic patients to decrease the morbidity and avoided for most asymptomatic patients.rnObjectivesrn1. To evaluate the bacteriological profile of patients with complicated UTIrn2. To ascertain the associated risk factors.&rnMaterials and Methods: The study included 1069 patients (869 from Nephrology, Urology and Endocrinology and 200 from ICU). The urine samples were processed by culturing on Blood agar and MacConkey agar. Antibiotic susceptibility of the isolates were tested using the Modified Kirby-Bauer disc diffusion method as per CLSI recommendations.rnResults: Bacterial pathogens were isolated from 556 (52.01%) cases. Insignificant growth and No growth were observed in 513 (47.98%) individuals. Escherichia coli (33.81%) was the most common etiological agent followed by Budding Yeast (19.78%), Pseudomonas aeruginosa (7.91%) and Staphylococcus aureus (5.93%). Imipenem was found to be the most susceptible against Enterobacteriaceae (100%) and Pseudomonas aeruginosa (100%). Vancomycin and Linezolid (100%) followed by Teicoplanin (96.4%) were the most sensitive agents for Staphylococcus aureus. 22.3% of the Staphylococcus aureus isolates were Methicillin resistant. Budding yeast (61.74%) was the most commonly isolated etiological agent in ICU patients.rnConclusion: The study highlights the importance of structural and functional abnormalities of the Genito-Urinary tract in the pathogenesis of complicated UTI. Urine culture and antibiotic susceptibility tests provided useful information in choosing effective and prompt therapy against bacterial pathogens and cure of infected cases.