Background: Hepatitis B infection among health care professionals can be prevented by strategies like vaccination, increasing awareness and following universal precautions. The present study was conducted on newly admitted medical students to evaluate their knowledge regarding HB disease and to know their vaccination status. This occasion was also used to motivate students to take HBV vaccine if not taken earlier. Methods: An educational interventional study was carried out on 141 newly admitted 1st prof MBBS students of Gandhi Medical College Bhopal for duration of 12 months, 1^st July 2016 to 30^th June 2017. 141 of the students of 1^st prof were enrolled for the study, to whom, education intervention was provided in the form of lectures using PowerPoint presentation followed by vaccination of the student for hepatitis –B. Pre and post intervention Data were collected using pretested questionnaire. Data was compiled using MS excel 2007and analysed using Epi info 7 software. Results: Only 55% of the students had good knowledge about disease and modes of transmission & prevention. Surprisingly half of them were unaware of high risk of transmission to health professional. Most of the students were unimmunized mainly due to lack of awareness and motivation. Conclusions: It is recommended for Hepatitis B vaccination for all unimmunized students who enter medical profession. The orientation and sensitization programme should be held to create awareness regarding HBV infection.