Background: The most susceptible people to infectious diseases in the work environment are healthcare professionals. The dental professional is repeatedly exposed to many microorganisms present in blood and saliva. As a consequence, the incidence of certain infectious diseases is higher among dental professionals than observed for the general population. The aim of this study, therefore, was to determine and compare the level of awareness about infection control procedures among junior and senior dental students at colleges of dentistry in Qassim Area. Materials and Methods: The current study is a cross-sectional survey conducted in Qassim province among undergraduate dental students who attended dental colleges in this area (one public and two private dental colleges). Data collection started on February 2015. Results: Out of the 345 participating students, 76.23 % were aware about the metods of transmission of hepatitis B virus, 91.67% of fifth year responded correctly. About 82.03% of the studied subjects knew that needle recapping minimizes the risk of injury during clinical procedures; most of them 97.22% were from fifth year level. Conclusion: Senior students reported a greatest level of awareness about infection control procedures in comparison to their junior counterparts.