Background: Since late 1980s, emergence of vancomycin (a glycopeptide) resistance has been observed among Enterococcal isolates worldwide. Glycopeptide resistance in Enterococci has been dealt with in a few studies in India. Aims and Objectives: (1) Studying antimicrobial resistance pattern among Enterococcus species and (2) Detection of vanA gene in vancomycin resistant Enterococcal isolates. Materials and Methods: A total of 145 consecutive Enterococcal isolates identified by conventional methods were included in the study. Antibiotic susceptibility testing was done by disk diffusion and MIC testing method. Vancomycin resistant Enterococcus (VRE) isolates were subjected to PCR amplification targeting vanA gene. Statistical analysis was done in Microsoft Office Excel 2007. Results and Conclusion: Penicillin resistance was found in most number of Enterococcus isolates. Vancomycin resistance was found in 6.6% of isolates, most (90%) of which were from hospitalized patients. Most of the VRE isolates were multi drug resistant. Among VRE isolates, 70% were Enterococcus faecium. Eighty (80) % of VRE isolates had vanA gene.