While performing various vascular diagnostic interventional procedures and graft surgeries knowledge of Profunda femoris artery is useful. Knowledge of its variation in origin and position do help to avoid various post-surgical complications, secondary hemorrhage and in some cases formation of pseudoaneurysms. Thirty lower limbs from formalin fixed adult human cadavers were dissected in the department of anatomy at LN Medical College & Research Centre, Bhopal, India. The distance of origin and position of Profunda femoris artery was measured by using thread, measuring tape and digital vernier calliper. In 100% cases it arise from femoral artery. In majority of the cases, distance of origin of Profunda femoris artery was found to be in the range of 31-40mm and position of origin was found to 50% as posterolateral and other 50% as posterior.