Introduction: Intravenous (IV) therapy is an important and unavoidable part of therapy in NICU. Catheter related infections (CRI) were associated with IV therapy where we uses IV catheters. In the United States of America CRI ranged from 6.4 to 8.3 episodes per 1,000 patient days in neonatal intensive care unit (NICU). The skills and techniques and number of attempts for putting IV line were deciding CRIs. Many studies showed educational intervention on nursing staff knowledge and to improve aseptic technique while performing IV cannulation in NICU Methodology: a prospective interventional study done in a tertiary care hospital from January to june 2017 study participants were nursing population working in hospital who were willing. The study conducted in two phases included pre and postintervention phases Results: The number of nurses who had high knowledge level about intravenous cannulation was just 20%, while after intervention this percentage improved to 90%. There was huge improvement in quality care element of intravenous cannula insertion after intervention.