Requisition for estimation of lipid profile comes as a routine biochemical parameter. The required specimen for analysis is a fasting blood sample. The fasting condition of an individual for clinical analysis of sample is defined as time atleast after 8 hour after last food intake. Many attempts have been carried to simplify the lipid analysis by conducting it on non fasting sample. Apart from fasting, certain precautions to be taken care with respect to posture of the patient, diet, smoking, alcohol and drug intake. This update addresses the issue with these pre analytical variables and the way to reduce the affect of these confounders.