Prevotella species are present in humans, often existing in the oral cavity, gastrointestinal tract, and vagina. In humans, they can act as an opportunistic pathogen. The present study entitled “Study of association of intestinal prevotella in new onset Rheumatoid Arthritis (NORA)” was carried out in Department of Microbiology, JSS Hospital from March 2016- March 2017. A total of 30 stool samples for NORA cases and 25 for healthy controls were included in this study. In NORA cases maximum number of patients were from the age group of 31-40. Samples from NORA cases were screened for RF and Anti CCP testing by ELISA. RF showed 17 positive cases whereas Anti CCP were positive for 7 cases. Anaerobic culture of stool samples was negative for P. copri in both NORA and Healthy controls.