Introduction: Urinary Tract Infections is second most common type of infection after upper respiratory tract infections affecting millions of people each year. Common conditions associated with UTI are pyelonephritis, urethritis, cystitis and these conditions are caused by wide range of microorganisms. So this study was done to isolate and identify most common bacterial flora causing UTI and to study their antimicrobial susceptibility pattern. Material &Methods: This study was done on all Urine samples received in Department of microbiology, National Institute of Medical Sciences and Research, Jaipur over a period of 5 months from July 2018 to November 2018. Samples were received and processed as per the standard guidelines and antimicrobial susceptibility testing was done according to the standard CLSI guidelines. Result: Out of 120 Urine Samples received in lab, 78 samples were culture positive with majority of samples from female patients than male patients. There was predominance of Gram Negative bacteria in causing UTI than Gram Positive bacteria. Escherichia coli wasthe most common organism causing UTI. Meropenem, Imipenem, Nitrofurantoin, Vancomycin, Linezolid showed great sensitivity pattern while Penicillin, Ampicillin and Cephalosporins showed high resistant pattern. Conclusion: Current study will be very helpful in initiating proper Empirical treatment therapy of patients suffering from UTI and thus it will be helpful in reducing the morbidity rate and mortality rate.