Introduction: Nosocomial infection is a most important health-care problem. Ventilator‑associated pneumonia (VAP) remains the major cause of hospital morbidity and mortality in Intensive Care Unit (ICU) patients. During ventilation, the endotracheal tube (ETT) provides a direct channel for pathogenic bacteria to enter the lower respiratory tract and lung parenchyma. Several organisms are accounted for causing pneumonia and the study is aimed to see their antimicrobial susceptibility pattern. Material & Methods: This study was done on all endotracheal aspirate and tube samples received in Department of microbiology, National Institute of Medical Sciences and Research, Jaipur over a period of 5 months from July 2018 to November 2018. Samples were received and processed as per the standard guidelines and antimicrobial susceptibility testing were done according to the standard CLSI guidelines. Result: There were 52 endotracheal tube/aspirate Samples received in lab out of which 41(78.85%) samples showed significant growth with the rest samples either no growth or polymicrobial. There was major bacterial isolates Klebsiella pneumoniae (31.71 %), Escherichia coli (19.52%), Staphylococcus aureus (17.07%), Pseudomonas aeruginosa (17.07%), Citrobacter koseri (12.19%), Acinetobacter spp. (2.4%). Meropenem, Piperacillin/tazobactum, Vancomycin, Polymixin B, showed great sensitivity pattern while Cefepim, Ceftriaxone, Penicillin and Ampicillin showed high resistant pattern. Conclusion: Current study will be very helpful in initiating proper Empirical treatment therapy of patients suffering from VAP and thus it will be helpful in reducing the morbidity rate and mortality rate.