Background: Urinary tract infection (UTI) is one of the most common infections in children. The incidence of UTI varies with the age and sex of the children. Fever may be the only symptom of UTI, especially in young children. Escherichia coli is the predominant pathogen in both the community-acquired urinary tract infection and hospital-acquired urinary tract infection. Drug resistance has been a common occurrence in infections among adults and elderly, but now it is frequently seen in children as well. Objective: To determine the bacteriological profile and susceptibility pattern of organisms causing UTI among children. Material and Methods: A total of 600 urine samples collected from the suspected cases of UTI were enrolled. A calibrated loop method (semi quantitative method) was used for the isolation of bacterial pathogens from urinary samples. Significant isolates were identified by conventional methods according to the standard laboratory protocol. The antibiotic sensitivity test was performed by modified Kirby Bauer disc diffusion technique as per CLSI Results: A total of 600 clinically suspected cases of urinary tract infection were enrolled in our study among that 216 (36%) were significant growth and 384(64%) were sterile. Out of total 216 positive cases in which 118 cases (54.6%) belong to Female and 98 cases (45.4%) belong to Male category and the majority of cases (57.4%) found in the age group of 4 to 6 years. Among all the positive cases, E. coli was the most prevalent 136 (62.9%). Gram negative organisms showed highest sensitivity to Nitrofurantoin (74.5%) while Gram positive organisms showed highest sensitivity to linezolid (100%). Conclusion: The constant evaluation of the antibiotic sensitivity pattern of UTI pathogens for commonly used antimicrobial agents in a particular environment should be carried out regularly.