Background: The commonly occurring infections in newborn are ophthalmia neonatorum, omphalitis, skin infections, candidiasis, and gastroenteritis. ^{1, 2,} Mothers are the first care providers for their newborn and adequate knowledge of the mothers on care of newborn is needed to reduce the infant mortality rate. ³ The aim of the present study was to assess the effectiveness of an information booklet on knowledge regarding prevention of neonatal infections among primipara mothers in a selected hospital at Mangalore. Materials and Method: A pre-experimental one group, pre-test post-test design was used for the study. Purposive sampling technique was used to select 30 primipara mothers from the post-natal and postoperative wards. The investigator assessed the knowledge using structured knowledge questionnaire and an information booklet was administered to the subjects and on the seventh day post-test was conducted with the same questionnaire. The collected data was analysed using descriptive and inferential statistics. Result: The mean post-test knowledge score (19.63) was significantly higher than the mean pre-test knowledge score (14.53). The computed ‘t’ value (14.70) was higher than the tabled value (t₂₉=2.05) at p<0.05 level of significance and was inferred that information booklet was effective in increasing knowledge of primipara mothers regarding neonatal infections. There was no significant association between knowledge of primipara mothers on neonatal infection and demographic variable