Background: Surgical site infections (SSI) are one of the most common postoperative complications and the most frequently reported nosocomial infections, causing significant morbidity and mortality thereby having increased burden on patient as well as hospital. With this background, we conducted this prospective study on 100 clinically diagnosed patients of surgical site infections admitted in Department of General Surgery in a tertiary care hospital in Odisha from August 2018 to August 2020. Methods: One hundred cases of postoperative wound infections were analysed. Swabs were collected from infected surgical wounds with all aseptic precautions and processed by the conventional microbiological methods. Results: The most common microorganism isolated on culture was E.coli (36 cases) followed by Pseudomonas (15 cases) and the most sensitive antimicrobial was Amikacin and most resistant antimicrobials are Cephalexin and Ciprofloxacin. Post-surgical wound infection was commonly encountered in emergency cases with dirty type of wound class (55%). Conclusion: Majority of the wounds were managed with local wound management and use of appropriate antibiotics based on culture & sensitivity. To treat various types of micro-organisms like E.coli and S. aureus which are responsible for surgical site infection, it is necessary to use antibiotics- (empirical & based on sensitivity profile). The most integral part is rational use of antibiotics which is necessary to combat the emerging multi drug resistant organisms.